Detection of <i>Pythium helicoides</i> in flower production area using PCR with species-specific primers.
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منابع مشابه
Specific PCR-primers for detection of Picoa lefebvrei desert truffle in Carex stenophylla roots
Picoa species are hypogeous desert truffles, which can be found in semi-arid ranges of North Africa, West Asian, South of Europe and Middle East, including Iran (Moreno et al. 2000, Jamali & Banihashemi 2012, 2013). Picoa species form symbiosis mainly on roots of annual and perennial herbaceous plants of the Helianthemum, including H. ledifolium and H. salicifolium var. salicifolium (Jamali & B...
متن کاملSpecific Primers For Identification and Detection of Three Major Desert Truffle Species in Iran with Special Reference to Terfezia claveryi
ABSTRACT- Desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. A PCR-based method was developed for the identification of 3 major desert truffles of Iran: Terfezia claveryi, Tirmania pinoyi and Tirmania nivea based on internal transcribed spacers of rDNA. Two specific PCR primers were designed for T. claveryi, 4 for T. pinoyi, and 2 for ...
متن کاملDetection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers.
Denaturing gradient gel electrophoresis (DGGE) of DNA fragments obtained by PCR amplification of the V2-V3 region of the 16S rRNA gene was used to detect the presence of Lactobacillus species in the stomach contents of mice. Lactobacillus isolates cultured from human and porcine gastrointestinal samples were identified to the species level by using a combination of DGGE and species-specific PCR...
متن کاملGenus- and species-specific PCR primers for the detection and identification of bifidobacteria.
16SrDNA-targeted genus- and species-specific PCR primers have been developed and used for the identification and detection of bifidobacteria. These primers cover all of the described species that inhabit the human gut, or occur in dairy products. Identification of cultured bifidobacteria using PCR primer pairs is rapid and accurate, being based on nucleic acid sequences. Detection of bifidobact...
متن کاملSelecting specific PCR primers with MFEprimer.
Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind to unintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA. In this chapter, we introduce: (1) the factors that affect the specificity of primers; (2) the principle of MFEprimer and its settings; (3) ...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 2007
ISSN: 0031-9473,1882-0484
DOI: 10.3186/jjphytopath.73.86